BIOL 44208 - DNA Sequencing Laboratory

Credit Hours: 1.00. In this lab module pairs of students prepare, run, and detect DNA sequencing ladders on miniaturized fast-running sequencing gels. Sequencing reactions are prepared from single-stranded and double-stranded DNA templates. The theory of DNA sequencing is taught, and experience is gained in manually reading the sequence from the sequencing ladders consisting of A, T, G and C lanes. Students learn how to connect to the National Center for Biotechnology Information (NCBI) and do BLAST sequence comparisons of the sequences that they read with DNA sequences in the national database GenBank. Typically offered Fall.

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BIOL 44208 - DNA Sequencing Laboratory