May 04, 2024  
2017-2018 University Catalog 
    
2017-2018 University Catalog [ARCHIVED CATALOG]

BIOL 44209 - Exploring The Living Cell


Credit Hours: 1.00. In this five-week module, students will learn how to observe and analyze cellular structure and dynamic behavior of living cells. We will utilize live cell imaging techniques to visualize molecules, cellular structures and organelles by use of fluorescence dyes and marker proteins tagged with green fluorescent protein (GFP) and other color florescent proteins. Specimens may include whole live organisms and cultured cells, as well as live tissues and organs from animal and plant systems. Students will observe the cytoskeleton and organelles in growing cells, perform bimolecular fluorescence complementation (BiFC) analysis to see molecular interactions in the live cell and monitor protein and organelle behavior during mitosis. Live specimens will be observed under stereo-dissecting, conventional, and confocal fluorescence microscopes. Images will be acquired, digitized, and analyzed by computational programs. Typically offered Fall.